<p>A) BHK-T7 cells were transfected or co-transfected for 2 h with 1 µg plasmid encoding HAV(IRES)-luc alone or in presence of 1 µg pTM1-2A or pFMDV-L, respectively. After 2 hpt, cells were treated with 200 µM Ars for 15 min and then metabolically labeled with 0.2 µCi per well [<sup>35</sup>S]Met/Cys in presence (+) or absence (−) of Ars for 45 min. Finally, cells were processed by SDS-PAGE, fluorography and autoradiography. B) The same samples were used to analyze eIF4GI, eIF2α phosphorylation and total eIF2α by western blot using specific antibodies as detailed in Materials and Methods. C) BHK-T7 cells were transfected under the conditions described above. Cells were then collected and processed to assay for luc activity as described in Materials and Methods. The bars represent the luc activity in presence (+) or absence (−) of Ars. The RLUs values obtained were as follows: pHAV-luc in absence (−) or presence (+) of Ars were 3.9×10<sup>5</sup> and 1.8×10<sup>5</sup>, respectively. pHAV-luc co-transfected with pTM1-2A (−) or (+) Ars were 0.2×10<sup>5</sup> and 0.1×10<sup>5</sup>, respectively, and finally pHAV-luc co-transfected with pFMDV-L (−) or (+) Ars were 25×10<sup>5</sup> and 17×10<sup>5</sup>, respectively. Error bars indicate standard deviation (SD). D) BHK-T7 cells were transfected with cap-luc, HAV(IRES)-luc or PV(IRES)-luc mRNAs. At 2 hpt cells were collected and luc activity was measured. The RLUs values obtained were as follows: cap-luc: 1.13×10<sup>6</sup>; HAV(IRES)-luc:1.46×10<sup>6</sup> and PV(IRES)-luc: 0.44×10<sup>6</sup>.</p
