<p>(A) Representative western blots of V5 (∼26 KDa), endogenous caspase-3 (∼35 KDa) and activated caspase-3 (∼19 KDa and 17 KDa) in Caco-2 cells. Preconfluent Caco-2 cells were transiently transfected with one µg of either GSTA1-V5 or empty vector (EV) for 48 h and treated with NaB (10 mM) for 48 h. β-actin (∼42 KDa) was used as a protein loading control. (B) Densitometric analysis of caspase-3 activation in GSTA1 over-expressed cells with and without NaB (10 mM) treatment. Values represent the mean ± S.E of three independent experiments. Bars indicated by different letters differ significantly from one another (p≤0.001).</p
