Expression of iron metabolism regulation genes in murine gastric mucosa.

Abstract

<p>Relative expression levels of genes involved in the regulation of iron metabolism were measured by qRT-PCR in biopsies of the gastric corpus mucosa of INS-GAS mice at 9 months post-infection with <i>H. felis</i> and in age-matched uninfected INS-GAS mice and FVB/N controls. The graphs represent the mRNA abundance of the test genes relative to the house keeping gene, <i>Gapdh</i>. Uninfected (<b>white bars</b>) (n = 10) and <i>H. felis</i> infected (<b>hatched bars</b>) transgenic INS-GAS mice (n = 10) were compared to the uninfected FVB/N genetic background control (<b>solid bars</b>) (n = 5). The relative levels of total hepcidin genes (<i>Hamp 1</i> and <i>2</i>) (<b>A</b>), Ferroportin 1 (<i>Fpn1</i>) gene (<b>B</b>), bone morphogenic protein gene 4 (<i>Bmp4</i>) (<b>C</b>) and 6 (<i>Bmp6</i>) gene (<b>D</b>) were assessed. Error bars represent mean ± SEM. Statistical analysis by unpaired <i>t</i> test with Welch’s correction.</p

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