<p><b>A</b>: Double labeling of TGF-β2 mRNA (black <i>in situ</i> hybridization signal) and immunoreactivity of the microglia marker Iba1 (brown precipitate). The lesion is indicated by star symbols (*) and the lesion border is demarcated by black dots. The positions of the high-magnification images in layer III and V of the cerebral cortex are indicated by black arrowheads as Aa, and Ab, respectively. Iba1-immunoreactive microglia do not contain TGF-β2 mRNA in either layer of the cerebral cortex. <b>B</b>: Double labeling of TGF-β2 mRNA (black <i>in situ</i> hybridization signal) and NeuN-immunoreactive neurons (brown precipitate). Almost all TGF-β2 mRNA-expressing cells contained NeuN immunoreactivity in layer III (Ba), and in layer V (Bb). Examples of double-labeled cells are indicated by black arrows. <b>C</b>: TGF-β2 is not expressed in astrocytes. TGF-β2 mRNA expressing neurons are indicated by white arrowheads and GFAP-immunoreactive neurons are indicated by black arrowheads. There are no double-labeled cells present. Abbreviations: cc – corpus callosum. Scale bars = 1 mm for A, 50 µm for Ab, 200 µm for Ba, 100 µm for Bb, and 100 µm for C.</p
