The general reaction scheme for <i>N</i>-ethylmaleimide on biological thiols.

Abstract

<p>The product formed is an <i>N</i>-ethylsuccinimido conjugate and the monoisotopic mass of the reactant is increased by 125.048. For example the glutathione (m/z 308.0911) conjugate is <i>N</i>-ethylsuccinimido-S-glutathione (m/z 433.1391).</p