Nuclear GFPVP26 aggregates do not sequester pUL36 and are not DNA replication compartments.

Abstract

<p>Vero cells were infected (inf.) for 9 h with 10 PFU/cell of HSV1(17⁺)blueLox (A) or HSV1(17⁺)blueLox-GFPVP26_{Δaa<b>1</b>–7} (B, D), or transfected (transf.) with the pHSV1(17⁺)blueLox-GFPVP26_{Δaa<b>5</b>–7} for 24 h(C, E). The cells were fixed with PFA and permeabilized with TX-100. GFPVP26 was detected by its intrinsic fluorescence. Furthermore, the cells were labeled with anti-pUL36_{aa1408–2112} and with MAb 5C10 against VP5 (A–C), against the single-strand DNA binding protein ICP8 and VP26 (D, E), or with TO-PRO-3 to stain the DNA (A). The arrows point to cytoplasmic capsids. Scale bar: 10 µm.</p