DXP levels in cells expressing wild type and mutant <i>aceE</i> and <i>ribB</i> genes.

Abstract

<p><i>E.coli</i> cells defective in both DXS and DXR were transformed with pCRII-TOPO constructs with the indicated wild type or mutant genes or an empty vector control (Ø). Positive transformants were grown in triplicate for 5 h with MVA, and DXP levels were measured by LC-MS. Mean and standard deviation (n = 3) values are represented relative to the levels in Ø controls.</p