<p>A: TGFβ1 reduced expression of mRNA IGFBP-1 in a time dependent manner, 48 h, p<0.01, 72 h, p<0.001. 1.0 = absolute value of ΔCT = 12. B: TGFβ1 inhibited release of IGFBP-1 protein release after only 2 h of treatment (p<0.001) and this continued to decline in a time-dependent manner (all time points p<0.001). C: TGFβ1 inhibited expression of PRL mRNA in a time dependent manner, 48 h (p<0.01), 72 h (p<0.001). 1.0 = absolute value of ΔCT = 11. D: TGFβ1 inhibited PRL protein release after only 2 h of treatment (p<0.05) and this was sustained for up to 72 h (12 h, p<0.01, 24 h, p<0.05, 48 h and 72 h, p<0.01). E: TGFβ1 inhibited expression of TF mRNA in a time dependent manner; 24 h (p<0.05), 48 h (p<0.001), 72 h (p<0.001). 1.0 = absolute value of ΔCT = 14. Data are mean ± S.E.M; * p<0.05, ** p<0.01, *** p<0.001 vs. control. n = 8 endometrial samples (in triplicate).</p
