Therapeutic effects of M-HIFU on RM-9 tumors.

Abstract

<p><i>A,</i> Ultrasound imaging guided M-HIFU treatment. Cavitation activities induced by M-HIFU within the tumor tissue are shown in regions with hyperechogenicity, indicated by arrows in the B-mode images (upper panel) and in the excised tumors (lower panel. Left: skin on top of the tumor was intact after M-HIFU treatment; middle: removed tumor showing intact exterior surface after M-HIFU treatment; right: bisected tumor showing mechanical lesions in the interior region after M-HIFU treatment. <i>B</i>, Tumor growth curve post rechallenge. 2×10⁴ RM-9 cells were subcutaneously injected into the contralateral hind limb 30 days after M-HIFU treatment when the mice were fully recovered from surgical resection of the primary tumor. Volume of the rechallenged tumors was calculated based on caliper measurements of tumor size at 3-day intervals starting from day 7 following tumor rechallenge. *p<0.05, **p<0.01. <i>C,</i> Cumulative survival curve following tumor rechallenge. Mice reaching the humane endpoint were sacrificed. There were 10 mice in the surgery group and 12 in the HIFU + surgery group. P-value of 0.019 was determined by the log-rank test. Data were obtained from three independent experiments.</p