<p>A, Alignment of residues 125-135 of human TEM4 with other species. Residues tested for actin binding <i>in vivo</i> are marked with a dot above the alignment. The arginine residue (R130), that we determined to be critical for binding to F-actin, is marked with the asterisk. B, Wild-type and point mutants of GFP-TEM4 1-582 were used to further map residues essential for actin association <i>in vivo</i> in live NIH3T3 cells. C, Coomassie Blue-stained gel comparing ATP/ADP-P<sub>i</sub>-F-actin binding capacity of wild-type (WT) and R130D mutant of GST-ABD in co-sedimentation experiments. D, Equilibrium binding of WT and R130D mutant of TEM4 ABD to ATP/ADP-P<sub>i</sub>-F-actin calculated from co-sedimentation experiments as shown in Fig. 4. Two independent co-sedimentation experiments were used to generate the data.</p
