<p>Epithelial cells at 14 days post-confluence were incubated with [<sup>35</sup>S]-methionine in the presence of PYY (1–36) (50 or 200 nM) and unlabeled oleic acid for 24 h to stimulate apo biogenesis. At the end of the labelling period, cells were washed, homogenized, and centrifuged. Supernatants from the cell homogenates were then reacted with excess antibodies for 18 h at 4°C to precipitate specific apos. Immune complexes were washed and analyzed by linear 4–15% SDS-PAGE. After electrophoresis, gels were sliced and counted for radioactivity. Data represent means ± SD for n = 3 separate experiments in each group. Values are reported as % of control values representing 100%. *P<0.05 vs. controls.</p
