<p>(A) The lysates of myc-Nkx2.5 and OGT-flag-co-transfected HEK293 cells were subjected to immunoprecipitation with anti-myc antibody and immunoblottings were performed with antibodies against <i>O</i>-GlcNAc (CTD110.6, RL-2) and myc. The modification of myc-Nkx2.5 with O-GlcNAc was detected. (B) Diabetic mice (DM) maintained elevated blood glucose level (>430 mg/dL) at 3 and 7 days after injection with streptozotocine (180 mg/kg body weight). The heart homogenates of control and diabetic mice were immunoprecipitated with anti-Nkx2.5 antibody, followed by immunoblottings with RL-2 antibodies against <i>O</i>-GlcNAc and anti-Nkx2.5 antibody. Nkx2.5 proteins of heart tissues were modified with <i>O</i>-GlcNAc and this modification increased in diabetic mice compared with control mice.</p
