<p>(<b>A</b>) CCR7 mRNA production analysis. Day 6 BMDCs were treated with LPS (1 µg/mL) and/or FTY720 (500 nM) for 24 h. Total mRNA were extracted, reverse transcripted and quantified by the real-time PCR. *<i>P</i><0.05, n = 3. (<b>B</b>) Nitric oxide production assays. Supernatants of cells with similar treatments were obtained and analyzed by diazotization reaction and colorimetry. *<i>P</i><0.05, n = 3. (<b>C</b>) Phagocytosis assay of BMDCs. Day 6 BMDCs were treated with LPS (1 µg/ml) alone or FTY720 (500 nM) alone for 24 h and subjected to fluorescence-conjugated beads uptake assays. Representative of results on the percentage of phagocytes were shown in each flow chart. Statistical analysis on the percentage of phagocytotic cells were presented as mean ± SEM. *<i>P</i><0.05, n = 3. (<b>D</b>) Endocytosis assay of BMDCs. Similar experimental design to (<b>C</b>) were employed. Cells were treated with FITC-conjugated dextran and analyzed by FACS. Representative results on the percentage of endocytotic cells were shown. Statistical data were presented as mean ± SEM. *<i>P</i><0.05, n = 3.</p
