<p>HA-Nak1 or HA-Orb6 were expressed in WT (SP199), <i>nak1</i> and <i>orb6</i> ts cells as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037221#pone-0037221-g001" target="_blank">Figure 1</a>. Cells were grown in thiamine free liquid PMAU media to an O.D. = 1.0 at 33°C and examined by DIC microscopy.</p