<p>Estimated total copy-number of <i>Leptospira interrogans</i> DNA recovered from a spiked sample of BACTEC™ anaerobic Plus containing approximately 50.000 <i>Leptospira</i>/5 µl by five DNA extraction methods. The mean copy-numbers are given above each column. Error bars indicate the 95% confidence interval. M1: Qiagen, DNeasy® Blood & Tissue Kit; M2: Molzym, MolYsis Plus Kit; M3: Protocol described by Fredricks and Relman; M4: Improved protocol for anaerobic and paediatric blood culture media; M5: Improved protocol adapted for all blood culture media.</p
