<p>Extracts from WT (SP199) cells expressing HA-Orb6 and control vector (lane 1), Myc-Nak1 and control vector (lane 2), HA-Orb6 and Myc-Nak1 (lane 3), HA-Orb6 <sup>S291A</sup> and Myc-Nak1 (lane 4), or HA-Orb6<sup>T456A</sup> and Myc-Nak1 (lane 5) from <i>nmt1</i> promoter expression plasmids were analyzed by western-blots using anti-Myc (9E10) or anti-HA (12CA5) monoclonal antibodies (Input panels). HA-Orb6 was immunoprecipitated from cell extracts with anti-HA antibody and equal portions of the immunoprecipitates were probed with anti-HA antibody and anti-Myc antibody (αHA IP panels).</p
