3D Sholl analysis of apical and basal arbors in YFP<sup>+</sup> L5 motor cortical neurons.

Abstract

<p>A) Confocal microscopic image stacks from 200 µm tissue sections showing YFP<sup>+</sup> dendrites in apical (upper) and basal (lower) compartments (WT male mouse). Scale bar = 50 µm. B) Representative 3D traces from a WT neuron. 10 µm Sholl radii were used in the basal compartment and 20 µm radii in the apical compartment. The pial surface is shown as a dotted line with the apical Sholl origin defined as a point 300 µm from the pial surface. Depth in the z-axis is color-coded.</p

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