The influence of FIR on the PI3K signaling pathway in HUVECs.

Abstract

<p>(A) FIR exposure-influenced phosphatase and tensin homolog (PTEN) activity. HUVECs were treated with vascular endothelial growth factor (VEGF) (10 ng/ml) or FIR exposure as indicated for 30 min. PTEN activity in each sample was detected by PTEN Malachite Green Assay Kit. The relative level of FIR-induced PTEN activity is shown as the mean±S.D. from six experiments. (B) Western blotting of PI3K p85. GAPDH was detected as a loading control. (C) FIR exposure-induced RNA levels of PI3K p85. HUVECs were pretreated with FIR exposure for 30 min, and then cultured for the indicated periods. The mRNA quantity of PI3K in each sample was detected by a qPCR with specific primers for the PI3K subunits p85α and p85β. The relative mRNA level of FIR-induced PI3K is shown as the mean±S.D. from six experiments. C, control. * <i>p</i><0.05 vs. the control without FIR exposure.</p