LPS-TLR4-MD-2 induced CXCR7 expression alteration in colorectal carcinoma cell line.

Abstract

<p>A, Reverse transcriptase (RT)-PCR analysis (TLR4 and MD-2) on RNA isolated from 8 human colorectal carcinoma cell lines. B, LPS induced time- and dose-dependent CXCR7 and CXCR4 protein expression alterations. SW480 and Colo 205 cell lines were incubated with LPS (500 ng/ml) in the presence or absence of PMB (500 µg/ml), representative flow cytometric analysis of CXCR7 expression alterations were showed. C, LPS exposure induced a significant CXCR7 expression increase in total RNA conten. D, Exposure of SW480 and Colo 205 cell lines to LPS (500 ng/ml) had no effect on CXCR4 expression.</p