A549 cells increase HβD2 gene expression in response to <i>M. abscessus</i> variants lacking GPL, but not the <i>M. abscessus</i> 390S variant expressing GPL.
<p>A549 cell monolayers were uninfected or challenged with <i>M. abscessus</i> variants 390R or 390S. In addition, some uninfected A549 cell monolayers were treated with IL1β or MALP-2 as controls for the ability of A549 cells to upregulate HβD2 gene expression. After 8 hours, HβD2 gene expression was quantified by real-time PCR. The results of real-time PCR are expressed as the relative fold increase in HBD2 gene expression over that of the untreated group and presented as mean +/− SD of measurements from the same experiment performed in triplicate. <b>*</b> 390S versus 390R and 390V; <i>P</i><0.05, <i>t</i>-test.</p
