Characterization of Ran transgenic mice.

Abstract

<p><i>A</i>, Tail DNA was extracted from transgenic mouse lines expressing Ran-WT, Ran T24N or Ran G19V in pancreatic β cells under RIP control, and PCR products were amplified using RIP-HA (<i>top</i>) or RIP-Ran (<i>bottom</i>) primers. (+) or (−) refers to Ran-positive or –negative genotype, or positive or negative control primers for the amplification reaction. M, molecular weight markers. <i>B</i>, Pancreas tissues extracted from the various mouse cohorts genotyped for the presence (+) or absence (−) of Ran transgenes were analyzed by Western blotting. 3T3, extracts from NIH3T3 cells transiently transfected with HA-Ran cDNA used as control. a-HA, antibody to HA. <i>C</i>: Pancreas or liver tissues were isolated from PCR-confirmed Ran transgenic mice, and analyzed by Western blotting. β-actin was used as a loading control.</p