Effect of <i>RAD51C</i> point mutations on the interaction with XRCC3 and RAD51B.

Abstract

<p>(A) Yeast two-hybrid assays were performed with XRCC3 in the DNA-binding domain vector and either wild-type or site-specifically mutated <i>RAD51C</i> in the activating domain vector. (B) Yeast two-hybrid assays were performed with RAD51B in the DNA-binding domain vector and the <i>RAD51C</i> variants in the activating domain vector. Results from liquid ONPG assays are the average of 5–7 different transformants performed in triplicate, with the standard error of the mean. * : <i>P</i><0.05 and ** :<i>P</i>≤0.001 using the student <i>T</i>-test.</p