Confirmation of plasmid pCLJ-Erm transfer from <i>C. botulinum</i> strain 657BaCT4 to strain LNT01.

Abstract

<p>(<b>A</b>) Ethidium bromide stained PFGE of <i>C. botulinum</i> strains: LNT01 (Lanes 1 and 7), wild type strain 657Ba (Lanes 2 and 8); 657BaCT4 (Lanes 3 and 9) and LNT01 transconjugants (pCLJ-Erm) (Lanes 4–6 and 10–12); Lanes 1–6, nondigested DNA samples; Lanes 7–12, <i>Xho</i>I digested DNAsamples. Lambda PFG Marker (Lane M), New England Biolabs. The position of the pCLJ plasmid is indicated with an arrow. Southern hybridization with: (<b>B</b>) the <i>ermB</i> probe and (<b>C</b>) the <i>bont/bvB</i> probe. PFGE conditions: 6V/cm, 12°C, 1–26 s pulse time, 24 h.</p