<p>Panel A: flow cytometry plot showing Annexin V staining of lymph node-homed pDC from a representative HIV-1 patient and control individual. Histogram plots were obtained by gating onto the live cell population as assessed by forward and side light scatter profile (not shown), and then onto the pDC population as identified by staining with anti-BDCA2 and anti-CD123 antibodies. Top density plot and gray area histogram: healthy control; bottom density plot and black line histogram: HIV-1 patient. Panel B: summary results for expression levels of Annexin V by lymph node-homed pDC of healthy controls (black bar; <i>n</i> = 11) and HIV-1 patients (white bar; <i>n</i> = 18). We determined the percentage of Annexin V positive pDC from all individuals in the two study groups (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011110#s2" target="_blank">Materials and Methods</a>). Panel shows the Mean and SEM values of the percent Annexin V positive pDC.</p
