Antibacterial activity of asCATH1 and 2 against three species of bacteria - <i>Y. ruckeri</i> (A), <i>V. anguillarum</i> (B) and <i>E. coli</i> (C).

Abstract

<p>Bacteria were grown to mid-log phase then incubated for 18 h with varying concentrations of the Atlantic salmon cathelicidins. LL-37 was included as a control for bacterial killing and <i>E. coli</i> ATCC 25922 was included as a control bacterium. Absorbance was read at 600 nm and percentage growth was calculated by comparison to the no inhibition control lane in the assay. The MIC was defined as 50% inhibition, which is indicated by a dotted line on each plot. Data shown are the mean ± SE of triplicate wells.</p

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