Calcium is involved in shikonin-medaited glucose uptake.

Abstract

<p>(<b>A</b>) Insulin and A23187 stimulated glucose uptake in L6 myotubes. Data are mean ± SEM of three experiments (<b>B</b>) Measurement of intracellular calcium levels by the fluorescent ratiometric Ca²⁺ indicator indo-1 following acute treatment with shikonin (1 µM) or insulin (1 µM). ***p&lt;0.001, *p&lt;0.05, one way ANOVA . Graph show mean delta value ± SEM of 15 experiments performed. Asterisks represent statistical difference as analyzed by one way ANOVA between basal and treated cells (*p&lt;0.05, *** P&lt;0.001). (<b>C</b>) Effect of BAPTA-AM on glucose uptake mediated by shikonin in L6 mytoubes. Cell were stimulated with shikonin for 2 h in the absence or presence of 5 µM 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester), BAPTA (BA). Graph show mean ± SEM of 3 experiments performed. Asterisks represent statistical difference as analyzed by one way ANOVA (*p&lt;0.05, **&lt;0.01).</p