<p>(a) Fibroblasts isolated from <i>Crh+/+</i> and <i>Crh−/−</i> dermis plated at an initial density of 8×10<sup>3</sup> cells/well for four days. * represents statistical difference (P<0.05) between genotypes. (n = 4 wells/treatment/experiment, at least 5 independent experiments). (b) Fibroblasts isolated from both genotypes cultured at an initial density of 10<sup>5</sup> cells/well in serum supplemented or serum free medium for 30 hr and apoptosis was measured by staining cells with Annexin V/Propidium Iodide and flow cytometry. The experiment was performed three times, with representative results of one experiment shown here.</p
