Sensitivity of moRK13 cell assay for the detection of RML mouse prions.

Abstract

<p>Serial 10-fold dilutions (from 10⁻⁴ to 10⁻⁸) of RML 10% brain homogenate were inoculated in duplicate to moRK13 cells and the inoculated cultures were proceeded as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020563#pone-0020563-g001" target="_blank">figure 1D</a>. PrP^res was analyzed by western blot after one (A) or two rounds (B) of cell assay. M are standard molecular mass marker proteins in kDa.</p