Baji Ádám

SZTE  Doktori Értekezések Repozitórium (SZTE Repository of Dissertations)

Summary of Ph.D. thesis SYNTHESIS OF STERANE-CONDENSED NITROGEN-CONTAINING HETEROCYCLES FROM BIFUNCTIONAL SEX HORMONE DERIVATIVES Baji Ádám Supervisor: Dr. Éva Frank Associate professor  Doctoral School of Chemistry University of Szeged Faculty of Science and Informatics Department of Organic Chemistry Szeged 20181  1. Introduction and aims Steroids with a four-ring framework are natural compounds found in living organisms. Their biological activity depends largely on the quality, position and spacial orientation of functional groups on the sterane core and on the stereostructure of the whole molecule. Today, one of the main driving force of steroid research is the production of semi-synthetic sex hormone derivatives which, as a result of modifications, lead to the reduction or elimination of the primary hormonal effect with the simultaneous appearance of a new, completely different bioactivity. Many previously synthesized heterocyclic steroidal compounds have been shown to inhibit the proliferation of tumor cells of diverse origins or have direct cytotoxic activity, therefore such modifications of sex hormones was one of the objectives of the present study. Based on the literature background and the synthetic results of the Steroid Chemistry Research Group, our aim was to produce new nitrogen-containing heterocyclic derivatives fused to the sterane skeleton. We designed the feasibility and the optimization of the conditions of each reaction on rings A and D of sex hormones. We also wanted to investigate the effect of substituents of the reagents on the ring-closure processes. Our aim was to determine the exact structure of the compounds and to subject all derivatives to in vitro pharmacological examinations in cooperation. 2. Experimental methods All of the reactions were performed in millimolar scale, and were monitored by thin layer chromatography (TLC). In addition to conventional heating, in some cases microwave (MW) irradiated reactions were also carried out in closed vessels. The crude products were purified by flash cromatography. The structure of each product was determined by different analytical methods (ESI-MS, IR, NMR).   2  3. Scientific results 3.1. Steroidal β-chlorovinyl aldehyde (2) was synthesized from estrone-3-methylether (1) by the Vilsmeier-Haack reaction (POCl3, DMF). The cyclization of the bifunctional steroid (2) with aniline (4a) under solvent-free conditions or in DMF afforded the desired sterane-fused quinoline (5a) in moderate yield (Scheme 1). 3.2. Similar transformations with p-toluidine (4b) and p-anisidine (4c) led to the heterocyclic products (5b, 5c) in moderate yields, while reactions failed with aniline derivatives containing electron-withdrawing groups (EWG) (Cl, NO2). Only certain intermediates, which underwent decomposition during the purification process, could be detected by TLC in these latter cases.  Scheme 1 3.3. For further experiments, 6-methoxytetralone (6) was used as a model of ring A of the sterane core. Non-steroidal β-chlorovinyl aldehyde (7) was prepared with the Vilsmeier-Haack complex, similarly to the synthesis of 2. The microwave-assisted reactions of 7 with aniline (4a) and substituted anilines (4b‒f) in DMF resulted in benz[c]acridine derivatives (9a‒f) in varying yields, depending on the steric and electronic character of the substituents on the aromatic moiety (Scheme 2). 3   Scheme 2 3.4. The microwave-assisted reaction of β-chlorovinyl aldehyde (11) (synthesized from 17β-acetoxy-5α-dihydrotestosterone (10) by the Vilsmeier-Haack reaction at room temperature) with aniline (4a) or substituted aromatic amines (4b‒i) in DMF afforded ring A-fused quinolines (13a‒i, 13g', 13i'). Ortho-substituted amines (4d, 4h) lowered the yields of the desired products (13d, 13h), while syntheses with meta-substituted aniline derivatives (4g, 4i) resulted in regioisomeric mixtures (13g and 13g' or 13i and 13i'). Electronic effect of the substituents was also observed during the reactions. Electron-donating groups on the reagents increased, while electron-withdrawing groups decreased the maximum amount of the heterocyclic compounds (Scheme 3).  3.5. During the reaction of 11 with 4-nitroaniline (4j) the formation of steroidal quinoline was not observed, a N-aryl-imino-enol product (15) was isolated instead. 3.6. The ring A-fused products (13a‒i, 13g') were deacetylated in alkaline (KOH) methanol to afford the corresponding 17β-OH analogues (14a‒i, 14g'). 4  Scheme 3 3.7. 16-hydroxymethylene-dehydroepiandrosterone (17) was next synthesized from dehydroepiandrosterone-3β-acetate (16) by the Claisen condensation. Compound 17 was treated with phenylhydrazine (18a) under general Knorr conditions in ethanol in the presence or absence of acetic acid catalyst leading to the regioselective formation of a single arylpyrazole (19a). Reaction of 17 with phenylhydrazine hydrochloride (18a·HCl) in pyridine also proved to be regioselective (19a), while a regioisomeric mixture of 19a and 20a in a ratio of 1:1 was obtained in refluxing ethanol in the presence of 0.3 equivalent amount of p-TsOH (Scheme 4). 5   Scheme 4 3.8. Catalytic reactions (0.3 equiv. p-TsOH) of 17 with p-substituted phenylhydrazine hydrochloride salts (18b‒h·HCl) in ethanol afforded the regioisomeric mixture of the products (19b‒g and 20b‒g) in different ratios, with the only exception of that with p-nitro-phenylhydrazine (18h) where the synthesis was regioselective (only product 19h). The ratio of 6  the regioisomeric pairs was shifted towards the formation of 19 over 20 in case of EWGs and 20 over 19 in case of EDGs (Scheme 4). 3.9. 2-Hydroxymethylene-5α-dihydrotestosterone (21) was also prepared from 17β-acetoxy-5α-dihydrotestosterone (10) by Claisen condensation. The reaction of 21 with phenylhydrazine hydrochloride (18a) or p-substituted phenylhydrazine derivatives (18b‒h·HCl) in acidic ethanol (0.3 equiv. p-TsOH) at room temperature led to the two possible regioisomers (22a‒h and 23a‒h) (Scheme 5).  3.10. Transformations of the bifunctional steroid derivative (21) with arylhydrazine hydrochloride salts (18a‒h·HCl) in pyridine at room temperature also resulted in the mixtures of regioisomers (22a‒h and 23a‒h), in contrast to the similar reactions on ring D of the sterane core. The major formation of 23 over 22 in case of strong EWG-containing (CN, NO2) reagents (18g, 18h) was observed. EDGs on the hydrazines did not have any significant impact on the isomeric distribution. 3.11. According to the observations, the formation of ring A-fused pyrazoles followed a different reaction mechanism than that of the ring D-condensed analogues. In the latter case, the formation of hydrazone intermediates followed by intramolecular cyclocondensation occurs, while two consecutive dehydration steps of the dihydroxypirazolidine intermediates may result in the ring A-fused pyrazole derivatives. 7   Scheme 5   8  3.12. The silica gel-supported, solvent-free, microwave-assisted multicomponent reaction of 16-hydroxymethyene-dehydroepiandrosterone (17), ammonium acetate and benzaldehyde (24a), or p-Cl-benzaldehyde (24b) (1:2:2 molar ratio) resulted in steroidal ring D-fused arylpyrimidine derivatives (25a, 25b) in sluggish yields (2025%). When the reactions were repeated using the same reactant ratios of the reagents in EtOH, the amount of the products was increased with 10% (Scheme 6).   Scheme 6 3.13. Similar transformation of 2-hydroxymethylene-5α-dihydrotestosterone (21), ammonium acetate and benzaldehyde (24a) in a molar ratio of 1:2:2 in EtOH under microwave irradiation afforded the ring A-fused pyrimidine derivative (26a) in a yield of 45%. After optimization of the reagent ratios (steroid:aldehyde:ammonium-acetate = 1:5:5), the yield of 26a increased to 60% (Scheme 7).  3.14. The syntheses of similar 2'-arylpyrimidines were also carried out under the optimized conditions by using different substituted benzaldehyde derivatives (24b‒i) in order to investigate the steric and electronic effects of the substituents of the reagents on the yields of the desired products (26b‒i). The greater the distance of the group R from the reaction center was, the higher yield of the heterocyclic compound could be obtained. EWGs facilitated the reactions, while EDGs were less favorable for the ring-closure step compared to the reaction of benzaldehyde. 9   Scheme 7 3.15. The microwave-promoted Biginelli-type reaction of 17β-acetoxy-5α-dihydrotestosterone (10), benzaldehyde (24a), and urea in a molar ratio of 1:2:1 in acetic acid in the presence of a catalytic amount of cc. H2SO4 afforded an inseparable mixture of dihydropyrimidine diastereomers (27a-4'R and 27a-4'S). The subsequent Jones oxidation led to a single pyrimidinone product (28a) (Scheme 8).  3.16. Reactions of 10, p-substituted aromatic aldehydes (24b‒g) and urea, applying similar conditions were also performed to investigate the substituent effect of the reagents to the cyclization process. We could not observe any substituent effect in the Biginelli-type reactions. 3.17. Deacetylation of the steroidal 2'-arylpyrimidinones (28a‒g) was carried out in alkaline MeOH to furnish 17β-OH analogues (29a‒g) in excellent yields. 10   Scheme 8   11  4. Scientific publications forming the basis of the thesis 1. Á. Baji, A. Gyovai, J. Wölfling, R. Minorics, I. Ocsovszki, I. Zupkó, É. Frank Microwave-assisted one-pot synthesis of steroid–quinoline hybrids and an evaluation of their antiproliferative activities on gynecological cancer cell lines RSC Advances 2016, 6, 27501 IF: 3,108 2. Á. Baji, F. Kovács, G. Mótyán, Gy. Schneider, J. Wölfling, I. Sinka, I. Zupkó, I. Ocsovszki, É. Frank Investigation of pH and substituent effects on the distribution ratio of novel steroidal ring D- and A-fused arylpyrazole regioisomers and evaluation of their cell-growth inhibitory effects in vitro Steroids, 2017, 126, 35.  IF: 2,282* 3. Á. Baji, T. Kiss, J. Wölfling, D. Kovács, N. Igaz, M. K. Gopisetty, M. Kiricsi, É. Frank Multicomponent access to androstano-arylpyrimidines under microwave conditions and evaluation of their anti-cancer activity in vitro Journal of Steroid Biochemistry and Molecular Biology 2017, 172, 79. IF: 4,561* Total IF: 9,951* MTMT ID: 10053209   12  5. Scientific lectures and posters forming the basis of the thesis Lectures: 1. Á. Baji, É. Frank Androsztánváz D-gyűrűjéhez kondenzált pirazolok előállítása mikrohullámú besugárzással 2015.04.29., Szegedi Ifjú Szerves Kémikusok Támogatásáért Alapítvány 14. tudományos előadóülése, Szeged 2. Á. Baji, É. Frank Szteránvázas heterociklusok előállítása multikomponensű reakciókkal 2016.11.08., A Magyar Tudomány Ünnepe ‒ Új kihívások a gyógyszerkémiában, Szeged 3.  Á. Baji, É. Frank Szteránvázhoz kondenzált nitrogéntartalmú heterociklusok szintézise bifunkciós nemi hormon származékokból 2017.11.27., MTA Szteroid- és Terpenoidkémiai Munkabizottsági ülése, Szeged.  Poster presentations: 1. Á. Baji, É. Frank, J. Wölfling, Gy. Schneider Efficient synthesis of steroidal ring A-fused quinolines by microwave irradiation 2014.09.07–10., 22nd Conference on Isoprenoids, Prague Chemicke Listy 2014, 108, s126.  2. Á. Baji, É. Frank, J. Wölfling, Gy. Schneider, I. Zupkó Microwave assisted synthesis of steroidal ring A-fused quinolines  2015.06.16–19., 16th Tetrahedron Symposium, Berlin (P2.058) 3. Á. Baji, É Frank, J. Wölfling Microwave-assisted synthesis of steroidal ring D-fused pyrazoles 2015.03–05., 5th Meeting of the Paul Ehrlich MedChem Euro-PhD Network, Cracow (P-2)   13  4. Á. Baji, G. Mótyán, É. Frank Pirazolgyűrűvel módosított androsztánvázas vegyületek mikrohullámú szintézise 2015.08.31–09.02., MKE 2. Nemzeti Konferencia, Hajdúszoboszló (GY-P-2) 5. Á. Baji, É. Frank, M. Kiricsi Androsztánvázas pirimidinek szintézise és in vitro hatástani vizsgálata 2017.06.19–21., MKE 2017. évi Vegyészkonferencia, Hajdúszoboszló (P-4) 6. Á. Baji, É. Frank, M. Kiricsi Multicomponent synthesis of novel androstano-pyrimidines and their pharmacological evaluation in vitro 2017.06.25–28., 10th Joint Meeting on Medicinal Chemistry, Dubrovnik (P-5) 7. É. Frank, Á. Baji, G. Mótyán, J. Wölfling, M. Kiricsi Ring A- or D-modified steroids with selective anticancer activity against androgen-independent prostate cancer cell lines 2017.09.10–14., 42nd FEBS Congress, Jerusalem (P-5.2.66) 6. Scientific publications forming the basis of the thesis 1. Z. Kádár, Á. Baji, I. Zupkó, T. Bartók, J. Wölfling, É. Frank É. Efficient approach to novel 1α-triazolyl-5α-androstane derivatives as potent antiproliferative agents Organic & Biomolecular Chemistry 2011, 9, 8051. IF: 3,696 2. G. Mótyán, Á. Baji, I. Zupkó, É. Frank Regio- and stereoselective synthesis of pregnane-fused isoxazolines by nitril-oxide/alkene 1,3-dipolar cycloaddition and an evaluation of their cell-growth inhibitory effect in vitro Journal of Molecular Structure 2016, 1110, 143. IF: 1,753 Total IF: 5,449 14  7. Scientific lectures and posters not forming the basis of the thesis Lectures: 1. Á. Baji 1α-Triazolil-5α-androsztán származékok előállítása intermolekuláris Cu(I)-katalizált cikloaddícióval 2013.04.04‒06., XXXI. Országos Tudományos Diákköri Konferencia, Eger 2. D. Kovács, J. Márton, Á. Baji, G. Mótyán, J. Wölfling, É. Frank Androsztánvázas 17-oxa-, és 17-tiadiazolok szintézise 2013.11.12., MTA Szteroid- és Terpenoidkémiai Munkabizottsági ülése, Budapest Poster presentations: 1. D. Kovács, G. Mótyán, Á. Baji, Gy. Schneider, É. Frank 1,2,4-oxadiazolgyűrűvel módosított androsztánvázas vegyületek szintézise 2013.06.26–28., Vegyészkonferencia, Hajdúszoboszló (P-31) 3. D. Kovács, G. Mótyán, Á. Baji, Gy. Schneider, É. Frank Efficient approach to steroidal 1,2,4-oxadiazoles in the androstane series 2013.06.30–07.04., VIIIth Joint Meeting on Medicinal Chemistry, Lublin  (P-23) 4. D. Kovács, G. Mótyán, Á. Baji, É. Frank, J. Wölfling Efficient approach to novel ring-condensed steroidal isoxazolines by 1,3-dipolar cycloaddition 2014.06.29–07.04., 20th International Conference on Organic Synthesis, Budapest (P-51) 5. Á. Baji, É. Frank, D. Kovács, L. Mérai Microwave-assisted access to novel steroidal 17β-3-pyrazole-5-ones 2015.06.07–10., IXth Joint Meeting in Medicinal Chemistry, Athens (P-50)   15  6. B. Molnár, G. Mótyán, Á. Baji, É. Frank Pirazolin- és triazolgyűrűvel módosított ösztron származékok előállítása mikrohullámú aktiválással 2017.06.19–21., MKE Vegyészkonferencia, Hajdúszoboszló (P-35) 7. G. Mótyán, Á. Baji, J. Wölfling, É. Frank, I. Zupkó Steroidal pyrazoles and pyrazolines as potent antfiproliferative agents 2017.09.10–14., 42nd FEBS Congress, Jerusalem (P-5.2.61) Σ IF: 15,400  

Szteránvázhoz kondenzált nitrogéntartalmú heterociklusok szintézise bifunkciós nemi hormon származékokból

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Szteránvázhoz kondenzált nitrogéntartalmú heterociklusok szintézise bifunkciós nemi hormon származékokból

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