<p>The relative avidity of anti-BoNT/A IgG serum antibodies was measured using day 162 serum using a modified ELISA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016532#s2" target="_blank">Materials and Methods</a>. <b>A</b>. The percent antibody bound in the ELISA in the presence of 3 M ammonium thiocyanate is indicated for individual serum samples that had measurable anti-β-trefoil IgG. A higher percent antibody bound represents a greater relative avidity for vaccine-induced, antigen-specific antibody. •: represents serum anti-β-trefoil IgG with no detectable BoNT/A neutralizing activity. ○: represents serum anti-β-trefoil IgG with detectable BoNT/A neutralizing activity. <b>B</b>. Average percent antibody bound when samples were organized by the neutralization capacity of the serum. Vaccine-induced BoNT/A neutralizing antibody has significantly greater avidity than non-neutralizing antibody. <b>a</b>: % antibody bound significantly greater than in the no neutralization group. Two-tailed Mann Whitney, p = 0.0239.</p
