<p>Differential Interference Contrast (DIC) (A, C, E, G, I, K, M, O, Q, S and U) and epifluorescence (B, D, F, H, J, L, N, P, R, T and V) images of wild-type (A, B, E, F, I, J, M, N, Q and R), <i>tbc-2(tm2241)</i> (C, D, G, H, K, L, O, P, S and T), and <i>tbc-2(tm2241) rab-7(ok511)</i> (U and V) oocytes (A–D) and different stage embryos (4-cell, E-H; bean stage at the beginning of morphogenesis, I-L; 1.5 fold stage of elongation, M-T; mid-stage of nonviable <i>tbc-2(tm2241) rab-7(ok511)</i> is late proliferative/early morphogenesis stage, U and V) carrying maternally deposited YP170::GFP from the integrated transgene <i>bIs1</i>. All images are of live animals except Q-T, which are fixed and immunostained with an anti-GFP antibody. Quantification of YP170::GFP fluorescence average intensity per unit area in wild-type and <i>tbc-2(tm2241)</i> proximal oocytes and bean stage embryos (<i>n</i> = 23 for both strains) (X and Y). A two-tailed unpaired Student t-test was used to determine statistical significance. n.s., not significant. ***, p<0.0001. Error bars represent standard deviations. Bars, 10 µm.</p
