LXR-deficient macrophages produce increased levels of NO following exposure to Leishmania and IFN-γ in vitro.

Abstract

<p>A. BMDM from individual wild-type and DKO (Sv129xC57Bl/6) mice were stimulated <i>in vitro</i> with stationary-phase <i>L. chagasi/infantum</i> promastigotes for the times indicated, in the absence (left) or presence (right) of IFN-γ pre-treament. B. BMDM (left) or peritoneal macrophages (right) from wild-type (solid bars) or LXR-DKO (empty bars) mice (C57Bl/6 background) were infected with stationary-phase <i>L. chagasi/infantum</i> (with or without IFN-γ pre-treatment) for 24 hours <i>in vitro</i>. Supernatants were removed and levels of NO were quantitated by the Griess method. Error bars represent standard deviations of triplicate NO measurements. Results are representative of at least 3 independent experiments; * denotes p<0.05, ** p<0.005.</p