<p>(A) Sequence alignment of the <i>gurken</i> transcription unit displayed using the Vista Browser at <a href="http://pipeline.lbl.gov/cgi-bin/gateway2" target="_blank">http://pipeline.lbl.gov/cgi-bin/gateway2</a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015448#pone.0015448-Nielsen1" target="_blank">[49]</a>. The estimated years in millions (MYA) of evolution between <i>D</i>. <i>melanogaster</i> and each of the other five species is from Heger and Ponting <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015448#pone.0015448-Heger1" target="_blank">[24]</a>. The most highly conserved region is circled and includes the first 39 nt of the GLS. The last 25 nt of the GLS map to the 3′ side of the abutting intron. The arrow indicates the direction of transcription. The red shaded region corresponds to a putative transposable element. The numbers at the bottom of the graph indicate nucleotide position along the chromosome. (B) The 5′ end of the <i>gurken</i> mRNA, where the green dot denotes the translation start site, the red arrows the boundaries of the GLS, and the asterisk the position of the intron. The nucleotides beneath the aligned sequence blocks highlight differences between the <i>D. Willistoni</i> and <i>D. melanogaster</i> sequences. (C) Predicted secondary structure of the GLS, with non-conserved residues shown in red.</p
