<p>Microtiter wells were coated with 50 µg/ml collagen I (<i>panel</i> A) or collagen III (<i>panel</i> B) and subsequently incubated with 0.1 µg/ml purified plasma-derived VWF in the presence of increasing concentrations of LAIR-1/Fc (open circles), LAIR-2/Fc (closed circles) or SIRL-1/Fc (squares). VWF binding was detected with horseradish-conjugated polyclonal anti-human VWF antibodies and 3-3′-5-5′-tetramethylbenzidine. Data are representative of 3 independent experiments.</p
