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The favourable impacts of Index-Based Livestock Insurance: Evaluation results from Ethiopia and Kenya

Abstract

<p><b>A.</b> The indicated Hhat constructs were expressed in and purified from 293FT cells as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011195#s2" target="_blank">Materials and Methods</a>. Aliquots of each purified protein were analyzed by SDS-PAGE and Western blotting using anti-Flag antibody. EV, empty vector. <b>B.</b> Each purified HhatHAFlagHis construct was incubated with Shh at the indicated concentration in the presence of 100 µM <sup>125</sup>I-iodo-palmitoyl CoA for 1 h at room temperature. <b>C.</b> Each purified HhatHAFlagHis construct was incubated with <sup>125</sup>I-iodo-palmitoyl CoA at the indicated concentration in the presence of 40 µM Shh. Shh protein bands were excised from dried gels and the amount of <sup>125</sup>I-iodo-palmitate incorporation was determined by gamma counting. Graphs represent the average of three experiments corrected for non-specific incorporation of <sup>125</sup>I-iodo-palmitate as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011195#pone.0011195-Buglino1" target="_blank">[11]</a>.</p

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