Examples of whole-cell recordings and subthreshold characteristics.

Abstract

<p>(<b>A and B</b>) Maximum intensity projections of 2-photon image stacks acquired during recording from two neurons, one ChAT⁺ (A) and one ChAT⁻ (B). In each, the recording pipette is visible to the right of the soma. (<b>C and D</b>) Voltage responses to −200, −150, −100, −50, 0, 50 and +200 pA current steps for 300ms. Dashed lines indicate 0 mV. C is from the ChAT⁺ neuron in A; D from the ChAT⁻ neuron in B. (<b>E</b>) Voltage-current curves for the ChAT⁺ (black, filled symbols) and ChAT⁻ (open, grey symbols) neurons in A and B, both showing anomalous rectification at potentials hyperpolarized to rest. (<b>F</b>) Sag ratio (steady state / peak voltage) during 300 ms, −200 pA current steps in ChAT⁺ and ChAT⁻ neurons.</p