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乌鳢(Channa argus)干扰素刺激基因Viperin和ISG15及其启动子的克隆与特征分析
Authors
周秀霞
贾伟章
郭琼林
黄容
Publication date
1 January 2007
Publisher
Abstract
应用抑制差减杂交、末端快速扩增和基因步移技术,克隆并鉴定了乌鳢干扰素刺激基因Viperin和ISG15及其启动子序列.Viperin cDNA全长1474nt,包含1059nt的开放阅读框,编码352个氨基酸.除N末端70个氨基酸外,Viperin蛋白氨基酸序列在鱼类和哺乳类具有高度的保守性.ISG15 cDNA全长758nt,包含468nt的开放阅读框,编码155个氨基酸,含有两个泛素样(UBL)结构域,C末端具有保守的UB偶联结构(LRGG).Viperin和ISG15启动子区存在保守的干扰素刺激反
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:152342/846
Last time updated on 15/03/2018