The mechanism of formation of the maxillary sinuses is not elucidated as yet, although their
morphology during embryogenesis is well described. In the prenatal period, the pneumatization hypothesis
is not valid. As the molecular approach to this problem is difficult to apply to human samples,
we decided to apply immunohistochemical reactions to analyse the synthesis of selected molecules
involved in the rebuilding of tissues. Hematoxylin-eosin staining and immunohistochemical
reactions for the detection of MMPs (matrix metalloproteinases), one of their inhibitor TIMP 1 (tissue
inhibitor of MMPs), BMP 6 (bone morphogenetic protein 6) and TGF-_ (transforming growth
factor _) were performed in the epithelium the mucosa of the maxillary sinuses of several human
foetuses from the collection of the Anatomical Institute. The age of the foetuses was 8, 11, 15, 16, 17,
18 and 22 weeks. An intense positive reaction for MMPs 1,2 and 3 was found in the mucosal epithelium
of developing sinuses in the whole series of foetuses was found. The reaction was more intense
in advanced stages of foetal development. Tissue derived inhibitor TIMP was hardly detectable, regardless
of the age of samples. However, the intensity of the reaction for TGF ß was strong in both
young and more mature sinus epithelium. The presence of BMP 6, a member of the superfamily of
TGF ß, was detected although the intensity of this reaction in the epithelium was rather weak. Both
TGFß and BMP 6 are well known as regulators of differentiation in the course of organogenesis.
Results of the histochemical analysis suggest the possible involvement of the epithelium in the
growth and formation of the maxillary sinuses. The main argument for this is intense reaction for
MMP proteases which, as in bone, regulate the turnover and rebuilding processes of the extracellular
matrix (ECM)