Improvement of determination on TTC - Dehydrogenase activity in biofilms

Abstract

The method of 3, 5, 5 - triphenyltetrazolium (TTC) determining biofilms activity was improved, and the problem that the standard curve of determination on TTC - dehydrogenase activity can't be made conveniently and stably was resolved. The factors which influence the determination were studied. The results showed that sodium sulfide as reducer, toluene as TF organic extractant, Tris - HCl as buffer solution, pH 8.6 and 38 degrees C were the best condition on the developed method, and the optimum reaction Lime to cultivate biofilms is 6 h