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research
大鳞副泥鳅7个群体遗传变异的微卫星分析
Authors
俞小牧
游翠红
童金苟
Publication date
1 January 2012
Publisher
Abstract
利用泥鳅(Misgurnus anguillicaudatus)的微卫星引物跨种扩增大鳞副泥鳅(Paramisgurnus dabryanus),通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167~4.833,平均观测杂合度(Ho)在0.248~0.417,平均期望杂合度(He)在0.379~0.505,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用UPGMA法对7个群体基于DA遗传距离进行聚类,可分为4类,位于长江中下游的沙市、澧县、武汉、鄱阳湖群体先聚为一类,后与石首群体聚类,最后与珠江水系的广州群体聚类,而位于长江上游的泸州群体则单独聚为一类。群体的F-统计量(Fst)为0.2987,表明群体间存在显著的遗传分化,主要由泸州群体与其它地区群体间的遗传分化引起
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:342005/17431
Last time updated on 15/03/2018