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research
银鲫果糖-1,6-二磷酸酶的分子克隆与表达分析
Authors
桂建芳
王锐
肖青
Publication date
1 January 2010
Publisher
Abstract
果糖-1,6-二磷酸酶(EC 3.1.3.11)是糖异生中的关键限速酶之一,在糖代谢中起重要作用。哺乳动物存在肝脏型和肌肉型两种果糖-1,6-二磷酸酶同工酶,分别由Fbp1和Fbp2编码。银鲫作为我国重要的经济养殖鱼类,尚无果糖-1,6-二磷酸酶基因的有关资料,其组织分布特征和胚胎发育模式亦不清楚。本研究采用RACE方法从银鲫原肠胚SMART cDNA文库中扩增了果糖-1,6-二磷酸酶基因的全长cDNA,其长度为1170 bp,编码337个氨基酸残基,多重序列比对和系统发育分析表明该基因为肝脏型果糖-1
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:342005/14222
Last time updated on 15/03/2018