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脂质体介导的鲫CAB细胞转化及转化细胞的核移植
Authors
赵浩斌,朱作言
Publication date
1 January 2001
Publisher
Abstract
采用脂质体法成功地使gfp基因转入鲫囊胚细胞株CAB细胞基因组,获得了具有G418抗性的细胞。以转化细胞为供体,银鲫卵为受体,核移植得到了转基因的囊胚和原肠胚;并发现4℃处理细胞24h能显著改善核移植胚胎的发育
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:152342/3166
Last time updated on 15/03/2018