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稀有鮈鲫脑芳香化酶cDNA片段的克隆与表达分析
Authors
曹梦西
杨玉慧
+4 more
江文波
王玉凤
胡炜
赵浩斌
Publication date
1 January 2009
Publisher
Abstract
芳香化酶(P450arom)是雌激素合成过程中的关键酶,在性别分化中起重要作用。鱼类存在卵巢性和脑型两种芳香化酶,分别由Cyp19a和Cyp19b编码。稀有鮈鲫作为我国特有的实验动物,尚无其芳香化酶的有关资料,其性别分化机制亦不清楚。本研究采用RT-PCR的方法以简并引物扩增了稀有鮈鲫脑型芳香化酶基因Cyp19b的部分序列,其长度为1070bp,编码357个氨基酸残基,具有典型的芳香化酶结构域。RT-PCR分析发现该基因主要在稀有鮈鲫的脑中表达,在性腺、肠、肾中也有表达;其在雌、雄脑中的表达差异不显著。
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:152342/216
Last time updated on 15/03/2018