In this comunication we report our progress regarding the isolation and characterization of new

antifungal metabolites from this strain of T. viride

Cabras, Anna Lisa

Evidente, Antonio

Maddau, Lucia

Marras, Francesco Salvatore

English

UnissResearch

SardiniaChem2008
GIORNATA DI STUDIO DEDICATA 
ALLA CHIMICA ORGANICA 
DELLE MOLECOLE BIOLOGICAMENTE ATTIVE
30 Maggio 2008,  Aula Magna della Facoltà di Scienze – Sassari
Comitato Scientifico:
Giampaolo Giacomelli, Univ. Sassari; Giovanna Delogu CNR Sassari; Salvatore Cabiddu, Univ.  
Cagliari; PierPaolo Piras, Univ. Cagliari
Comitato Organizzatore:
Andrea Porcheddu, Univ. Sassari; Roberto Dallocchio, CNR Sassari;
Stefania De Montis Univ. Cagliari
Sponsor
hanno contribuito alla realizzazione del convegno:
UNIVERSITA’ di Sassari-Dipartimento di Chimica; UNIVERSITA’ di Sassari-Facoltà di Scienze 
MFN; CNR-Istituto di Chimica Biomolecolare, Sassari; UNIVERSITA’ di Cagliari;
SAPIO s.r.l.; SIGMA-ALDRICH s.r.l.; CARLO ERBA Reagenti;
MEDINLAB s.r.l.; VWR International s.r.l.
NH
OH
O
OO
Me
OH
O
Me
O
O
Me
OH
O
O
Me
O
O
Me
Me
H
O
P 20
ANTIFUNGAL METABOLITES PRODUCED BY TRICHODERMA VIRIDE AGAINST 
SCLEROTIUM ROLFSII
L. Maddau  1 , A. Cabras  1 , F. Marras  1  and A. Evidente  2 
1Dipartimento di Protezione delle Piante, Università di Sassari, 07100 Sassari, Italy
2Dipartimento di Scienze del Suolo, della Pianta e dell’Ambiente, Università di Napoli Federico  
II, 80055 Portici, Italy
Corresponding author: L. Maddau; Phone: +39.079.229368; 
As a part of a long-running project searching for fungi suitable for biological control of soil-
borne plant pathogens, we found a strain of Trichoderma viride that showed in vitro and in vivo 
antagonistic  activity  towards  Sclerotium  rolfsii,  the  causal  agent  of  crown  and  stem  rot  in 
artichokes. The noteworthy antagonistic activity exhibited by  Trichoderma spp. strains may in 
part  be  explained  by  the  production  of  different  classes  of  bioactive  metabolites,  including 
antibiotics such as peptaibols, inhibitors of fungal growth and enzymes.
In this comunication we report our progress regarding the isolation and characterization of new 
antifungal metabolites from this strain of T. viride.
Four pure compounds have been isolated up to now from the ethyl acetate extracts of the culture 
filtrates of T. viride. 
One of these, obtained as colorless liquid with a strong coconut-like odor, was a well-known 
fungal metabolite and was identified by means of its spectroscopic properties as 6-n-pentyl-2H-
pyran-2-one.
The second compound had previously been isolated and characterized as isoharziandione, a new 
tetracyclic diterpene capable of inhibiting fungal growth of S. rolfsii .
The  third  compound  was  isolated  as  a  homogenous  oil  resistant  to  crystallization  and  was 
characterized  as  a  new  6-substituted  2H-pyran-2-one,  trivially  named  viridepyronone.  The 
compound  had  a  good  antifungal  activity  against  S.  rolfsii,  and  its  minimum  inhibitory 
concentration (over 90 % inhibition) was found to be at 196 µg/mL. This was the first time that 
this metabolite had been reported as being produced by a fungal species.
72
P 20
Recently  from  the  same  culture  filtrate  of  this  fungus,  we  isolated  a  new  pentasubstituted 
oxiranyldecene,  named  viridenepoxydiol,  showing  inhibitor  effect  on  mycelial  growth  of  S.  
rolfsii. Its minimum inhibitory concentration (over 90 % inhibition) was found to be 396 µg/mL.
The results  of these studies provide new information on the production  in vitro of antifungal 
metabolites by  T. viride commonly employed as a possible agent for biocontrol in agriculture. 
Future investigation should be addressed to clarify the role of these metabolites in the biocontrol 
process and the nature of interaction between them or with enzymes.
73


Antifungal metabolites produced by <i>Trichoderma viride</i> against <i>Sclerotium rolfsii</i>

Abstract

Similar works

Full text

Available Versions

UnissResearch