Different methodologies are being developed, such as imaging, spectroscopy and
electrochemistry, to study neurochemical dynamics in cell cultures or in intact brain [1-2]. One of
these techniques involves the in-situ detection of biologically active molecules, including nitric
oxide (NO) [3], glucose [4], glutamate (GLUT) [5-6] and lactate [1,7], in brain extracellular fluid
(ECF), using implanted microsensors and biosensors. NO is a water-soluble free radical that
readily diffuses through membranes and its actions in the CNS are largely studied
