Additional file 1: of Effects of a wide range of dietary forage-to-concentrate ratios on nutrient utilization and hepatic transcriptional profiles in limit-fed Holstein heifers

Abstract

Table S1. Ingredient and chemical composition of experimental diets. 1 OM, organic matter; CP, crude protein; EE, ether extract; NDF, neutral detergent fiber; NFC, nonfiberous carbohydrate; ME, metabolizable energy. 2 Pre-experimental diet. 3 Contained 18.50% Ca; 6.00% P; 4.2% Mg; 1.4% K; 2.6% S; 7.5% Na; 12.0% Cl; 30 mg/kg of Se; 0.25% Zn; 0.25% Fe; 0.25% Mn; 1100 mg/kg Cu; 15 mg/kg I; 265,000 IU/kg vitamin A; 110,200 IU/kg vitamin D; and 2300 IU/kg vitamin E. 4 NFC = 100 − (NDF + CP + ether extract + ash). 5 Estimated as ME = total digestible nutrients × 0.04409 × 0.82, according to NRC (2001). Table S2. PCR primers for qRT-PCR validation of five randomly selected genes. Table S3. Nutrient intake of Holstein dairy heifers fed diets containing differing forage levels. Table S5. Gene Ontology analysis of total differentially expressed genes. Table S6. Differentially expressed genes clustered in Short Time-series Expression Miner (STEM) profiles 17, 19, and 4. (DOCX 71 kb