<p>(A–D) Cardiomyocytes were cultured for 24 h under basal conditions or with the addition of ISO/ICI and/or KH7 (12.5 μmol/L). In (A) the cross sectional areas of 118 cells are given (n = 5). In (B) the RNA (ng/μL)/DNA (ng/μL) ratio (n = 3) and in (C) the protein (μg/μL)/DNA (ng/μL) ratio are presented (n = 3). (D) Western blot analysis followed by optical band density analysis of α-skeletal actin (n = 5). Data were normalized to GAPDH band density and to the control (no treatment, 100%). (A-D) Data are expressed as means ± SEM. *(P < 0.05) or ***(P < 0.001) vs. ISO/ICI-treated cells. (E) Isolated rat cardiomyocytes were incubated with ISO/ICI and KH7 (0.0, 6.0, 9.0, 12.5, 15.0 μmol/L) for 24 h. The protein (μg/μL)/ DNA (ng/μL) ratio was determined (n = 4 cardiomyocyte preparations). Data are expressed as means ± SEM. 6.0 and 9.0 μmol/L KH7 vs 0.0 μmol/L KH7: P<0.05; 12.5 and 15 μmol/L KH7 vs 0.0mKH7: P<0.01. n refers to the number of cardiomyocyte preparations.</p
