<p>(A) Western blot analysis of phosphorylated CREB (pCREB,) from the lysates of untreated (-KH7) or KH7-treated (+KH7, 12.5 μmol/L) cardiomyocytes followed by optical band density analysis at different incubation times with ISO/ICI (n = 4). Data were normalized to the total proteins by gel staining as indicated in Methods; incubation times: 0, 15 min, 30 min, 1 h, 3 h, 6 h. Solid line indicates control, broken line—treatment with KH7. Western blot analysis of (B) phosphorylated B-Raf (pB-Raf; Ser445) and B-Raf (n = 3), of (C) phosphorylated Erk1/2 (pErk1, pErk2; TEY motif)) and Erk1/2 (n = 4) followed by the optical band density analysis (lower panel) are presented. Data were normalized to total protein and are expressed as means ± SEM. *(P<0.05), vs. ISO/ICI treated control cells. n refers to the number of cardiomyocyte preparations analyzed.</p
