IC1 methylation in <i>H19</i>^{<i>+/hIC1</i>Δ<i>2</i>.<i>2</i>} and <i>H19</i>^{<i>+/hIC1</i>} mice at E15.5.

Abstract

<p>(A) See legend to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007243#pgen.1007243.g004" target="_blank">Fig 4A</a>. (B) Percent methylation measured by pyrosequencing at two CTCF target sites (CTS1 and CTS6) from embryo body and placenta of <i>H19</i>^{<i>+/hIC1</i>Δ<i>2</i>.<i>2</i>} and <i>H19</i>^{<i>+/hIC1</i>} mice at E15.5 (left panel). The endogenous mIC1 was analysed as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007243#pgen.1007243.g004" target="_blank">Fig 4</a>. Each histogram represents the methylation mean value of 5 (CTS1 and mIC1) or 6 (CTS6) CpGs, tested in two litters of each KI: +/hIC1Δ2.2 (n = 6), <i>+/+</i> (n = 5); +/hIC1 (n = 6) and <i>+/+</i> (n = 4) embryos. Bars represent the mean ± SEM. (C) IC1 methylation analysed by bisulphite treatment followed by cloning and sequencing in embryo body and placenta of <i>H19</i>^{<i>+/hIC1</i>Δ<i>2</i>.<i>2</i>} and <i>H19</i>^{<i>+/hIC1</i>} mice at E15.5. See legend to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007243#pgen.1007243.g004" target="_blank">Fig 4</a> for more details.</p