A One-Step Chemoenzymatic Labeling Strategy for Probing
Sialylated Thomsen–Friedenreich Antigen
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Abstract
Abnormal
expression of sialylated Thomsen–Friedenreich antigen
(Neu5Acα2-3Galβ1-3GalNAcα-O-Ser/Thr, sialyl-T) has
a strong relationship with various types of human cancers and many
other diseases. However, the size and structural complexity, and relatively
lower abundance of sialyl-T have posed a significant challenge to
its detection. Therefore, details about the role of sialyl-T in a
variety of physiological and pathological processes are still poorly
understood. Here, a one-step chemoenzymatic labeling strategy to probe
sialyl-T is described. This approach enables the sensitive, selective,
and rapid detection of sialyl-T, and global profiling and identification
of unknown sialyl-T-attached glycoproteins, which are potential therapeutic
targets or biomarkers. The use of one-step labeling strategy not only
has a higher sensitivity than a typical two-step reporter strategy
but also avoids undergoing an additional chemical reaction step to
introduce a reporter group after the labeling reaction, making it
particularly useful for detecting low-abundance glycan epitopes on
living cells